Zoology

Download Animal Cell Technology: Basic & Applied Aspects: Proceedings by Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji PDF

By Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya (eds.)

ISBN-10: 1402096453

ISBN-13: 9781402096457

Animal cellphone know-how is a turning out to be self-discipline of cellphone biology which goals not just to appreciate constructions, features and behaviors of differentiated animal cells, but additionally to examine their skills for use for business and scientific reasons. The objective of animal telephone know-how comprises the clonal growth of differentiated cells, the optimization in their tradition stipulations, modulation in their skill to provide proteins of scientific and pharmaceutical importantance, and the appliance of animal cells to gene treatment, man made organs and the creation of practical meals. This quantity offers the readers an entire overview of the current cutting-edge and should be beneficial for these operating in both educational environments or within the biotechnology and pharmaceutical sectors, relatively mobile biologists, biochemists, molecular biologists, immunologists, biochemical engineers and all different disciplines with regards to animal phone culture.

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Read Online or Download Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006 PDF

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Extra resources for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006

Example text

1 × 104 cells/ml and after thawing, they were cultured in RPMI 1640 medium supplemented with FBS. (a) The number of viable cells was counted using the trypan blue exclusion method. Cryopreservation was performed in the presence of sericin (closed circles) and FBS (closed squares). 5 0 FBS Sericin Cryopreservative solution Fig. 2 Cell proliferation and antibody production after thawing. 0 × 104 cells/ml and after thawing, cells were cultured at 37°C in ASF103 medium. (a) The number of viable cells was counted using the trypan blue exclusion method.

Then, we demonstrated CHO cell lines transfected with ­ras-­oncogene Target gene (AE6F4) vector c-Ha-ras, dhfr gene vector Marker protein (CD4) vector Co-transfection into CHO-dhfr(-) and CHO-hp1 FACS cell sorting MACS (MA gnetic Cell Sorting) MTX (Gene amplification) Limiting dilution cloning Single cell sorting 1~2 weeks 1~2 days Low productive and weak clones Highly productive clones and rapid cloning CHO-dhfr(-) 0 clones/1000 seeded wells CHO-hp1 188 clones/1000 seeded wells Fig. 1 The method of the establishment of highly productive cell line 28 T.

We maintained the environment of a sample chamber as same as a general cell culture and succeeded in in-situ observation of the cell growth on the Si prism. The result of the MIR-IRAS measurement shows spectral enhancements at amide-I and amide-II absorption bands due to the cell growth. 2 Experiment Figure 1 illustrates the equipment made of Teflon® we used in this study for MIRIRAS measurements. This equipment was also used in our past studies [3, 4]. The volume of the sample solution was 100–200 ml.

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